Antiseptic and Disinfectants Preparations
-Antiseptics and disinfectants are prepared by diluting a specific amount of concentrated solution with distilled water.
-A pipet or measuring cylinder is used to measure the amount in milliliters of concentrated disinfectant.
-Usually, a measuring cylinder is used to measure the amount of distilled water added to dilute the measured amount of concentrated disinfectant.
-Conical flasks are used to mix the diluted disinfectant.
Material and Equipment can be needed In Preparation of Antiseptic and Disinfectant
- Beaker
- Measuring Cylinder
- Pasteur pipette
- Glass rod
- Distilled water
- Personal Protective Equipment
- SOPS
- Reagents Stocks
- Analytical Balance
- Spatula
DISINFECTANT PREPARATION
10% bleach (Jik®)
Is prepared by mixing 10mls of concentrated bleach with 90mls of distilled water to make 100mls.
Such that;
10mls Conc. Bleach.
90mls Distilled water.
-After mixing, the solution should have a label put on (tape with marker) to identify as 10% bleach with preparation date and technician name.
2% bleach solution
Is also sometimes used for antiseptic hand-washing after handling infective material.
This is prepared by diluting 2 parts (ml) of concentrated bleach with 98 parts (ml) of distilled water (2+98 = 100 total parts (mls)).
Then after mixing, the solution should have a label put on (tape with marker) to identify as 2% bleach with preparation date and technician name.
ANTISEPTIC PREPARATION
The same process is used to prepare diluted antiseptic, such as methylated spirits, to make 70% methylated spirits.
Prepared by mixing 70mls of concentrated methylated spirits with 30mls of distilled water to make 100mls.
Application of Antiseptics and Disinfectants.
Procedure for disinfection of glassware.
-Add concentrated bleach to a pan of tap water (approximately 10%) and add rinsed glassware or plastic ware and soak overnight (at least 8 hours).
-Rinse well in tap water, rinse in distilled or filtered rainwater, and air dry.
Procedure for Clothing and Rubber (reusable gloves).
-Soak in 1% bleach overnight before washing with detergent water.
Procedure for routine disinfection of laboratory bench.
-Wipe with a clean cloth soaked in 10% bleach or 5% Lysol.
-Wipe with a clean cloth to remove remains.
Procedure after spillage on a laboratory bench or other surfaces.
-Wipe with a clean cloth soaked in 10% bleach or 5% Lysol.
-Allow sitting for 10 minutes.
-Wipe with a clean cloth or mop soaked with detergent water to remove remains.
Procedure for Routine Hand Washing:
-Procedure: Antiseptic Handwashing:
- Wash with soap and water for 2 minutes.
- Wash with 2% bleach solution or 2 % Lysol solution.
- Air dry or dry with a towel.
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